draft date March 2002

Construction Zone!! This is a rough draft!!


Breaking News!

Meters can now be quickly and easily used to measure flesh pH.  Here is a link to a homemade holder for a pH meter to leave one hand free while testing.

Read all about it!
Since 1997, we have produced annual documents describing out chalky halibut investigations.  You can link to them  in PDF format here:



Background

Pacific halibut is fished by setline (long strings of line with attached hooks) on the continental shelf from northern California to the Bering Sea. The fishery currently operates eight months each year, from mid-March through mid-November. Chalky halibut has been recognized for decades, although until recently the problem was limited to summer fisheries in the southern fishing areas off Washington and Oregon. Until 1995, landings in most areas have occurred during short fisheries in the early and late summer, and most product was frozen. Since 1995, fisheries occur throughout the open period, and a majority of the product is sold fresh. This, combined with an increased awareness of chalkiness by the marketplace, has created the current situation, where as much as two million pounds of the 60-million pound catch is graded as chalky and thus unmarketable, constituting a multi-million dollar loss to the industry.

IPHC research, both in the 1960s and in the last five years, have shown chalkiness to be directly associated with a buildup of lactic acid and resulting lowered pH in post-mortem flesh. The condition is specifically associated with a denaturation of muscle proteins resulting in an increased drip loss and a sometimes startling loss of translucence in the flesh. In extreme cases, the flesh gapes, and has little use as a food product. Our research has associated chalkiness with two areas of the coast during late summer and early fall, and male halibut tend to be chalkier than females. It is likely that the areas with the highest rate of chalkiness are associated with high bottom temperatures (12-14 degrees C), which are near the upper thermal limit for the species’ distribution. Most recently, we have facilitated the use of pH meters in 1-2 day post-mortem fish to determine flesh pH, which is predictive of the developing chalky condition.

The condition is reversible in live fish. Flesh which would otherwise likely be chalky does not develop the condition post-mortem if the fish are allowed a 1-2 day resting period after capture, and before killing.
 

What is Chalky Fish?

Chalky fish are opaque in color, rather than translucent. Chalky flesh loses water more easily than normal (high "drip loss") Chalky flesh has a high level of acidity (low pH) The cooked flesh is dry and fibrous. Flesh appearance may lead to rejection by consumers.

Description of "chalky" halibut · flesh is "soft and flabby", has a dull, flat, ‘chalky’, opaque white color, contrasted to the firm, shiny, semi-translucent white flesh of non-chalky fish. · on cooking, chalky halibut is dry and fibrous compared to non-chalky. · chalky condition varies in degree, both between fish and within a single fish.

Time Progression of chalky development

Time progression of chalkiness · Chalkiness not apparent visually when landed on vessel. · Chalkiness develops post-mortem. · Flesh of ‘chalky’ fish is indistinguishable from that of normal fish at time of capture, at least in terms of visual appearance or protein extractability. · change to the chalky condition is dependent on the muscle pH. · No visual difference will be noticed in the time period 1 to 7 hours. A difference will be noticed in the time period 2 to 10 days. · The flesh loses its elasticity after the first week in iced storage · Chalkiness may develop more quickly or to a greater degree in fish held at higher temperatures.

Physiological changes associated with chalky fish

Physiological changes in chalky fish · The visual appearance of chalky fish is caused by a change in the state of muscle proteins, specifically, a decrease in the extractability of muscle proteins. · chalky fish are equal or superior to non-chalky fish nutritionally. Oil and protein content is higher and water lower in chalky fish. (Baily, 1951). · The dry weight of muscle tissue increases during iced storage of chalky fish more so than in non-chalky fish. This is paralleled by increases in relative protein content. · tendency to lose water (Patashnik, USBCS, unpub), both before and after cooking. · flesh elasticity during first week on ice better or equal to non-chalky, but subsequently deteriorates at greater rate (Patashnik, unpub). · the myotomes of chalky fish tend to separate more readily than non-chalky. · Chalky fish have a lower pH (higher acid level) than non-chalky fish. Fish with pH over 6.3 are never chalky. With pH under 6.0 are always chalky. With pH between 6.0 and 6.3 are sometimes chalky. · Chalkiness may not affect the entire fish. It may be more prevalent in the middle of the fletches, near the blood line.

Discussion

"Most fish are captured by methods that involve struggling, and consequently the free glucose and glycogen content of their muscles is usually very low. Most of the glycogen has already been degraded to lactic acid" Tarr, 1968.

The flesh of ‘chalky’ fish is indistinguishable from that of normal fish at time of capture, at least in terms of visual, pH, or protein extractability. The change to the chalky condition is dependent on the muscle pH. Acidity levels (pH) are directly related to levels of lactic acid in the flesh. Lactic acid is a byproduct of the conversion of glycogen into energy. Buildup of lactic acid is associated with fatigue. Rest after exertion results in a decrease in lactic acid levels. A fish which dies immediately after a high activity period will have high levels of lactic acid (low pH) and is likely to be chalky. (BUT: lactic acid is formed from glycogen postmortem: Tarr, 1968). A rest period, such as lying on the hook after capture, will lower lactic acid levels and reduce the chances of chalkiness. Well fed fish would have high energy reserves (glycogen). These fish may exercise more strongly at capture, producing higher lactic acid levels. Chalkiness is more frequent in trawl caught fish. These fish would have died after or been subject to extreme exhaustion.

Lactic acid theory · well-fed or heavy-feeding fish with high glycogen energy reserves · extreme physical activity causes large accumulation of lactic acid (fatigue byproduct) · removal of lactic acid is impeded or blocked · the higher the holding temperature, the more intense the condition and the more rapid its development. · resultant change in muscle proteins · NOTE: this is similar to a condition in rabbit, pig, beef, or whale meat. A well fed animal killed under physical struggle or stress conditions had similar meat degradation.

Experimental results · Trawl caught fish, brought on board dead, were tagged. At landing, visual and pH observations were recorded. Of 113 fish, 56 were ‘slightly chalky’ and 57 were ‘very chalky. Observations of pH of dorsal slab were made (Myhre, IPHC, notes) · <3 hr set, longline fish were not stunned immediately, 8 % of 88 fish were chalky (rep by Patashnik) · Tomlinson et al (ref 1) did an extensive experiment with trawl caught and some longline caught fish. · Freezing fish immediately after capture and dressing, on thawing flesh is indistinguishable for first 2 hours, then goes opaque. This change in appearance was accompanied by a decrease in pH and in protein extractability. · There was a tendency for the muscle pH or longline fish to be higher than trawl fish at time of landing on vessel. · Tomlinson (ref 3) allowed trawl-caught but still alive halibut to recover in a holding tank for 10 to 13 hours. Flesh was stores 11 days on ice. After storage, flesh from recovered fish had higher pH, and lower percentage were chalky (46 vs 77% and 0 vs 33%).
 
 

References

Bell, F. H. 1950. Notes regarding "milky", "mushy", and "chalky" halibut. Unpublished. International Fisheries Commission. Dated September 1950.

Kramer, D.E. and B.C. Paust. 1985. Care of halibut aboard the fishing vessel. Alaska Sea Grant college program, marine advisory bulletin no. 18. 30p.

Mannan, A., D. I. Fraser, and W. J. Dyer. 1961. Proximate composition of Canadian Atlantic fish. J. Fish. Res. Bd. Canada (18), p 483-493.

Myhre, R. J. 1968? Observations on chalkiness of halibut caught by the trawler New Washington on goose Island ground in July, 1968. Unpublished. 4 pages. IPHC.

Patashnik, Max. 1965. The problem of chalky halibut. Unpublished release, Technological Laboratory, U.S. Bureau of Commercial Fisheries, Seattle, Wa. Dated Feb. 18, 1965.

Patashnik, M. and H. S. Groninger, Jr. 1964. Observations on the milky condition in some Pacific coast fishes. J. Fish. Res. Bd., Canada, 21(2), p 335-346.

Pegg, R. 1966. Personal communication from Ron Pegg, Memorial University of Newfoundland, to Steve Kaimmer, IPHC.

Stromme, G. 1996. Personal communication from Gail Stromme, quality assurance person for Sitka Sound Seafoods, to Sheri Gross. 5p.

Tarr, H. L. A., 1968. Postmortem degradation of glycogen and starch in fish muscle. J. Fish. Res. Bd. Canada, 25(8): 1539-1554.

Thompson, W. F. 1916. A note on a sporozoan parasite of the halibut. IN: report of the commissioner of fisheries for the year ending December 31rst, 1915. Victoria, BC. P. 127-129,

Tomlinson N., Geiger, S.E., and E. Dollinger. 1964. Chalky halibut. Fish. Res. Bd. Canada. Vanc. Lab. Circ. No. 33, 8p.

Tomlinson N., Geiger, S.E., and E. Dollinger. 1965. Chalkiness in halibut in relation to muscle pH and protein denaturation. J. Fish. Res. Bd., Canada, 22(3), p 653-663.

Tomlinson N., Geiger, S.E., and E. Dollinger. 1965. Free drip, flesh pH, and chalkiness in halibut. J. Fish. Res. Bd., Canada, 22(5), p 673-680.

Tomlinson N., Geiger, S.E., and E. Dollinger. 1966. Influence of fishing methods on the incidence of chalkiness in halibut. J. Fish. Res. Bd., Canada, 23(6), p 925-928.

Unk. 1996. Chalky halibut, the fish buyer’s nemesis. The Westcoast Fisherman, April 1996, p 21-22.